Introduction to High Performance Liquid Chromatography and it’s parts

高效液相色谱（HPLC）是一种流行且通用的技术，可为复杂有机样品的分离，识别和定量提供负担得起的解决方案。

At first sight the assembly of complex modules and bunch of tubings might scare you. However, on gaining an insight into the role of each component the mystery will start unfolding and the HPLC system will turn out to be a user-friendly piece of equipment that if properly looked after will ensure highest accuracy and reliability of results analysis after analysis.

高性能液相色谱介绍的功率点呈现是对HPLC组件的简要简短介绍。重要的是要了解各个组件部分以及每个组件如何贡献分析的总体可靠性。您提高的舒适度将使您意识到HPLC是一种易于使用的系统，并具有无限应用的希望。

In the last slide of this HPLC presentation you will find useful links on an advancedHPLC证书课程。我们邀请您加入该计划，并借此机会通过参与不时举行的讨论论坛和网络研讨会来与我们的专家互动。

Introduction to High Performance Liquid Chromatography and its parts

Chromatography equipment looks rather intimidating to anyone who has not handled it before, but on a closer look and as you get familiar with the equipment you realize that behind the network of wires, complex plumbing and circuitry is a simple machine with only a few major parts.

这些部分的不同组合，即：

• 泵
• 探测器
• 喷油器基于应用程序产生无限数量的配置。

Just like an understanding of human anatomy makes you conscious of the vital role of each and every body organ towards your well being and vitality. Similarly you need to have a good understanding of the parts of your HPLC system to generate data of highest reliability.

A conceptual understanding of the function of each component will add to your comfort level with your HPLC system. You will ensure long time usage with high reliance on output data.

什么是HPLC？

HPLCis a technique for separation, identification and quantification of components in a mixture. It is especially suitable for compounds which are not easily volatalised, thermally unstable and have high molecular weights.

移动阶段

The liquid phase is pumped at a constant rate to the column packed with the stationary phase. Before entering the column the analysis sample is injected into the carrier stream.

到达色谱柱时，根据分析物分子和固定相之间的物理化学相互作用选择性保留样品成分。

移动阶段

流动阶段用于将样品运输到系统。流动阶段的基本标准是对样品成分的惰性。纯溶剂或缓冲液组合通常使用。流动相应没有颗粒杂质，并在使用前脱气。

流动相位库

These are inert containers for mobile phase storage and transport. Generally transparent glass bottles are used so that so as to facilitate visual inspection of mobile phase level inside the container. Stainless steel particulate filters are provided inside for removal of particulate impurities in the mobile phase if any.

泵

样品成​​分的流动相效应时间流量的流量变化并导致错误。泵在恒定压力下提供流动相恒定流向色谱柱的流动。

喷油器

喷油器用于向流动相流提供恒定体积注入样品。必须进行惰性和注射的可重复性，以维持高度的准确性。

Column

A column is a stainless steel tube packed with stationary phase. It is a vital component and should be maintained properly as per supplier instructions for getting reproducibility and separation efficiency run after run.

Column Oven

分析过程中温度的变化会导致分离洗脱组件的保留时间变化。柱烤箱使用空气循环保持恒定的色谱柱温度。这样可以确保通过列的流动阶段的恒定流速

Detector

A detector gives specific response for the components separated by the column and also provides the required sensitivity. It has to be independent of any changes in mobile phase composition. Majority of the applications require UV-VIS detection though detectors based on other detection technique are also popular these days.

数据获取与控制

Modern HPLC systems are computer based and software controls operational parameters such as mobile phase composition, temperature, flow rate, injection volume and sequence and also acquisition and treatment of output.

专业版本

专业的HPLC系统还可能具有溶剂选择阀，真空抽度器，自动采样器，列开关，列前或后列衍生化和分数收集器。这些都在我们在HPLC上的在线证书计划中详细介绍。HPLC证书课程

What Next?

• Keep Learning to Keep Succeeding – visitwww.m.drakkus.com/hplc要了解有关HPLC的更多信息
• 接受HPLC的在线证书培训 -www.m.drakkus.com/product/join-our-certificate- course-on-hplc/
• 订阅我们的新闻通讯，并直接提供免费培训到您的收件箱 -www.lab-training. com
• 没有HPLC？需要测试样品访问www.testing-lab.comand get a proposal!