HPLC固定阶段的进展：单片HPLC柱

Stationary phases forHPLC.have evolved from irregular particles to spherical particles to sub- 2μm size particles and核 - 壳技术。在整体包装的发展中已经看到平行进步。

单线可以可视化为“实心摇滚”，如没有间隙空间的结构。这些提供更短的扩散距离和用于溶质分散的多种途径。多孔棒状结构是高度渗透性的，并且与基于颗粒的固定相比相比，提供大的表面积进行分离。单片柱是基于二氧化硅的或具有有机聚合物骨架。

Historical

Richard Synge in 1952 conceptualized monolith as a continuous block of porous gel. The idea picked up in 60s and 70s when polyurethane foams were introduced. It was only in the late 1980s that hydrophilic gels and silica rods were developed.

Advantages over particulate phase HPLC columns

• 具有高渗透性的单片提供大量流动通道和高接触区域。这些功能提供了优于颗粒固定阶段的几个优点。
• Reduction of particle size is accompanied with increase in column backpressure and you are forced to use more expensive UHPLC systems. Backpressure problems are easily overcome when using monolith HPLC columns.
• 由于这种分子在颗粒柱中的姿势背部并发症中，因此全身是适用于大分子的分离
• 原位聚合的整体柱避免柱子变异性以及由于墙壁效应引起的移动流动变化被最小化。
• 与颗粒填充柱相比，整料HPLC柱更坚固，并且表现出机械稳健性。
• 颗粒包装柱需要溶剂环境，并且任何暴露在空气中导致干燥，这会导致无法弥补的损坏。整体列没有遇到这样的问题。
• 由于抗冲击性，提高耐温性和承受更宽的pH范围介质的能力，单金属柱HPLC柱具有延长的有用的生命周期。

主要应用领域

由于众多的优点庞然大物高效液相色谱columns hold promising scope for applications in biotechnology covering specialized fields such as genomics, proteomics, metabolomics and pharmacogenomics. Fast response and high resolution of large molecules has made possible chromatographic real-time monitoring of fermentation processes. Pharma, foods, forensics and clinical studies are other areas where monolith columns will hold promise in the coming time.

Nano HPLC is another area where monolith columns are having a future potential. Permeability of monolith packing circumvents the backpressure problem encountered with sub 2-μm packings and it is possible to use very low ID columns of the order of 20 μm with long column lengths.

Suppliers of monolith HPLC columns

BIA分离首先引入了聚合物整体HPLC Coloumns，然后用铬硫离产品系列开发和生产的二氧化硅整料。随后将产物线传递到2005年的肉豆蔻线.Dionex开创性的聚合物整料和目前的一些来源通过以下链接访问：

• 安捷伦
• Phenomenex
• Sigma Aldrich
• Thermo Scientific

整体列在未来具有很大的承诺。如果您已经接触到此类列，请分享您的经验并留下您的评论。