气相色谱（GC）在薄层色谱上manbetx官方的优点（TLC）

Chromatography is a separation science which has made remarkable contributions to advances in fields such as pharmaceuticals, foods, nutraceuticals, environmental monitoring, forensics, cosmetics and life sciences. The potential for applications are enormous and new ones are being reported on an impressive scale.

从简单的柱色谱到的多年来，色谱从简单的柱色谱到纸张色谱法薄层色谱和仪器技术，如manbetx官方andHPLC.。

本文将专注于气相色谱对薄层色谱技术的优点。manbetx官方

决议程度

manbetx官方气相色谱法具有解析诸如石油级分或烟雾的复杂混合物的组分，其在单个样品中可以包含数百或甚至更多的组分

使用TLC技术几乎不可能这样的复杂分辨率。

改善解决方案

Freedom to control operational parameters such as temperature programming, carrier gas flow rate, adjustment of split ratios can help resolve closely appearing peaks. It is not easy to resolve closely appearing or overlapping bands or spots in薄层色谱法技术。

Speed of analysis

即使在色谱运行期间也要改变操作参数非常方便，以在最短的时间内实现最佳分辨率。在板开发阶段，此选项不可用薄层色谱法。使用气相色谱法的分析通常以几分钟的时间manbetx官方内完成，而TLC色谱分离所需的时间几分钟

广泛选择样品

Gas chromatography is ideally suited for analysis of volatile compounds. Samples with boiling points as high as 380 to 400°C can be analysed by temperature programming. The samples can range from liquids, gases and dissolved solids. TLC techniques, however, cannot be used for analysis of gases.

Fully Quantitative Technique

GC is a fully quantitative technique with software capable of providing accurate data such as peak area counts for quantitative estimations. The TLC technique requires subsequent treatment of the spots such as dissolving in suitable solvent or extraction for subsequent spectrophotometric determinations. Accessories such as densitometers provide high accuracy but these require additional cost.

高灵敏度

manbetx官方气相色谱法提供全系列探测器，如火焰电离检测器（FID），导热探测器（TCD），电子捕获探测器（ECD），氮磷检测器（NPD），质量选择性检测器（MSD），火焰光度检测器（FPD）对于具有杂种原子如卤素，氮气，磷和硫的特异性化合物提供非常低的检测限。在TLC测定上不可能进行这样的检测水平

软件能力

manbetx官方气相色谱软件能够进行归一化，峰值和基线优化，这导致实际改善和报告的实际结果。在TLC分离期间，不可能实现参数的这种实时控制。

选择列

GC columns are available over a range of diameters and lengths ranging from little over 1 m to several meters (packed and capillary columns) along with a wide range of solid and liquid support phases. This offers a wide choice of applications for different types of separations

存储分析记录

Permanent storage of chromatograms is possible both as hard and soft copies. On the other hand TLC plates cannot be stored indefinitely due to fading of spots with time and requirement of proper storage conditions. It is, however, possible to take pictures of developed plates and maintain permanent reports but this involves extra cost

非破坏性测试的选择

GC offers a large choice of detectors some of which like TCD and FPD are nondestructive in nature. The output stream after detection on these detectors can be led to more sensitive detectors such as MSD or FT-IR.

Derivatization

Gas chromatography offers the option of derivatization of the original compounds through reactions such as silylation, alkylation and acylation for improved sensitivity of detection.

列重用

Gas chromatography columns do not require to be discarded after every single analysis as in case of thin layer chromatography where plates need to be replaced after each and every analysis. However, it is essential to take the recommended precautions on use and storage as advised by the column manufacturer and keep a check on the theoretical plate count from time to time. The column will require reconditioning or replacement if the number of plates falls below a specified value.

HPLC如GC，优于TLC的若干优点，这将在后续物品中占用。